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American Society of Microbiology ABMM Sample Questions:

1. A patient with a history of diabetes mellitus develops a severe foot ulcer. Deep tissue cultures grow multiple anaerobic bacteria, including Clostridium perfringens. The MOST concerning virulence factor produced by this organism in this context is:

A) Tetanospasmin
B) Alpha-toxin (lecithinase)
C) Botulinum toxin
D) Hyaluronidase

2. A patient with HIV infection and a CD4+ T-cell count of 30 cells/µL develops progressive multifocal leukoencephalopathy (PML). The causative agent of PML is:

A) Human herpesvirus 6 (HHV-6)
B) JC virus (JCV)
C) Epstein-Barr virus (EBV)
D) Cytomegalovirus (CMV)

3. A patient develops a severe diarrheal illness after returning from a backpacking trip where they drank untreated stream water. Stool examination reveals motile trophozoites with multiple flagella and a prominent ventral sucking disk. The MOST likely causative agent is:

A) Cyclospora cayetanensis
B) Cryptosporidium parvum
C) Giardia lamblia
D) Entamoeba histolytica

4. A clinical microbiology laboratory is performing antimicrobial susceptibility testing using the broth microdilution method. The minimum bactericidal concentration (MBC) is determined by:

A) Subculturing the clear wells from the MIC assay onto antibiotic-free media and determining the lowest concentration that results in no bacterial growth.
B) The highest concentration of antibiotic that allows visible growth after 24 hours of incubation.
C) The lowest concentration of antibiotic that inhibits visible growth after 24 hours of incubation.
D) Comparing the zone of inhibition around antibiotic disks to standardized tables.

5. During an outbreak of norovirus gastroenteritis in a nursing home, environmental swabs are collected to identify the source of the infection. Which of the following methods would be MOST appropriate for detecting norovirus in these samples?

A) Reverse transcription-polymerase chain reaction (RT-PCR)
B) Enzyme-linked immunosorbent assay (ELISA) for viral antigens
C) Culture on human embryonic kidney (HEK) cells
D) Gram staining and microscopy

Solutions: