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American Society of Microbiology ABMM Exam Syllabus Topics:

American Society of Microbiology ABMM Sample Questions:

Question 1

A clinical laboratory manager is implementing a new automated blood culture system. Which aspect is LEAST likely to be part of the initial verification/validation process before clinical use?

A. Establishing workflow and training competency for laboratory staff
B. Verification of detection of a panel of common bloodstream pathogens
C. Comparison of time-to-detection with the previous system
D. Assessment of the system's contamination rate over one year of routine use

Question 2

A microbiology laboratory receives a bronchoalveolar lavage sample from a patient with suspected ventilator- associated pneumonia. Quantitative culture yields >10^5 CFU/mL of a Gram-negative rod. Identification reveals Stenotrophomonas maltophilia. The MOST significant resistance mechanism commonly associated with this organism is:

A. Target site modification in DNA gyrase.
B. Intrinsic resistance mediated by efflux pumps and specific enzymes.
C. Ribosomal methylation leading to macrolide resistance.
D. Production of extended-spectrum beta-lactamases (ESBLs).

Question 3

A clinical microbiology laboratory is performing antimicrobial susceptibility testing using the broth microdilution method. The minimum bactericidal concentration (MBC) is determined by:

A. Subculturing the clear wells from the MIC assay onto antibiotic-free media and determining the lowest concentration that results in no bacterial growth.
B. The highest concentration of antibiotic that allows visible growth after 24 hours of incubation.
C. The lowest concentration of antibiotic that inhibits visible growth after 24 hours of incubation.
D. Comparing the zone of inhibition around antibiotic disks to standardized tables.

Question 4

A patient develops a severe diarrheal illness after consuming raw seafood. Stool culture yields a Gram- negative, comma-shaped bacterium that is oxidase-positive and grows on thiosulfate-citrate-bile salts-sucrose (TCBS) agar, producing blue-green colonies. The MOST significant virulence factor contributing to the severe, cholera-like diarrhea caused by this organism is:

A. Type III secretion system (T3SS)
B. Heat-labile toxin (LT)
C. Cholera toxin (CT)-like enterotoxin
D. Heat-stable enterotoxin (ST)

Question 5

A clinical microbiology laboratory encounters a Gram-negative bacillus isolated from a bloodstream infection. The isolate exhibits resistance to multiple antibiotics, including carbapenems. Further phenotypic testing reveals the production of a metallo-beta-lactamase (MBL). Which of the following antibiotic combinations might still retain activity against this isolate?

A. Piperacillin-tazobactam
B. Aztreonam-avibactam
C. Ceftazidime-avibactam
D. Aztreonam

Solutions: